Deciphering the Genetic Mechanisms of Salt Tolerance in Sorghum bicolor L.: Key Genes and SNP Associations from Comparative Transcriptomic Analyses

Sorghum bicolor L. is a vital cereal crop for global food security. Its adaptability to diverse climates make it economically, socially, and environmentally valuable. However, soil salinization caused by climate extremes poses threat sorghum. This study aimed identify candidate salt-tolerant genes single nucleotide polymorphisms (SNPs) performing comparative transcriptome analysis on mutant sorghum line its wild type. The was generated through gamma ray exposure selection salt tolerance. Phenotypic measurements were taken, followed mRNA sequencing variant calling. In this study, potential non-synonymous SNPs associated with tolerance inferred, including LOC8071970, LOC8067721, LOC110430887, LOC8070256, LOC8056880. These demonstrated notable differences in nsSNPs comparison the type, suggesting their roles Additionally, LOC8060874 (cyanohydrin beta-glucosyltransferase) suggested as key gene involved due possible role dhurrin biosynthesis under stress. upcoming research, additional reverse genetics studies will be necessary order verify function of those relation conclusion, underscores significance investigating mechanisms Our findings may provide insights future breeding strategies at enhancing salinity productivity.